Role of glutamate receptor-6 in kainate-induced epilepsy in rats
- VernacularTitle:谷氨酸受体-6在海人藻酸致痫大鼠发病机制中的作用
- Author:
Xiaomei LIU
;
Wei SUN
;
Xiaocui LI
;
Yafeng SUN
;
Renxian TANG
;
Dongsheng PEI
;
Guangyi ZHANG
- Publication Type:Journal Article
- Keywords:
kainate;
epilepsy;
glutamate receptor-6 (GluR6);
MLK3
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2010;31(2):156-159
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the molecular mechanism of glutamate receptor-6 (GluR6) in the pathogenesis of epilepsy. Methods Seizure model of SD rats was induced by intraperitoneal injection of kainate (KA). Immunoprecipitation and immunoblotting were performed to examine the interactions of GluR6 and MLK3 with PSD95 at various time points after KA injection. The effect of Tat-GluR6-9c on the MLK3 phospharylation induced by kainate was observed with immunoblotting and immunohistochemistry. Results The assembly of GluR6 and MLK3 with PSD95 was induced after KA hippocampal CA3 region, and bagan to decrease one day later. Pretreatment after KA injection in CA3 region (P<0.05). Conclusion KA induces the assembly of the GluR6-PSD95-MLK3 signaling module and subsequently activates MLK3, which ultimately results in brain injury.
