Protein expression of TIMP3 and RUNX3 in bone marrow mononuclear cells from acute leukemia patients
- VernacularTitle:急性白血病患者骨髓细胞中TIMP3、RUNX3蛋白表达情况检测及其临床意义
- Author:
Ruifang FAN
;
Zhigang FANG
;
Xiangfu LIU
;
Yongjiang ZHENG
;
Binbin LIU
;
Dongjun LIN
- Publication Type:Journal Article
- Keywords:
Protein TIMP3;
Protein expression;
Methylation;
Leukemia
- From:
Chinese Journal of Pathophysiology
2010;26(2):293-296
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To detect the protein expression of TIMP3 and RUNX3 in bone marrow mononuclear cells (BMMCs) from acute leukemia (AL) patients and to investigate the relationship between the methylation status of genes and their expressional levels. METHODS: Protein expression of TIMP3 and RUNX3 in 50 samples of BMMCs and 10 samples of peripheral blood mononuclear cells (PBMCs) from healthy volunteers was detected by Western blotting. The prognostic factors related to AL and data from methylation specific polymerase chain reaction were also analyzed. RESULTS: The expression level of RUNX3 with methylation was less than that without methylation in BMMCs from AL patients. The complete remission (CR) rate was related to RUNX3 expression and blasts in bone marrow (BM). BMMCs from patients with silencing of RUNX3 and higher blasts in BM had a lower CR rate. CONCLUSION: Absence of RUNX3 protein expression resulting from methylation of RUNX3 promoter probably plays a role in the pathogenesis of AL and is of value in prognosis. No relationship between methylation of TIMP3 promoter and the pathogenesis of AL is observed.
