Arsenic trioxide inhibits KM3 cell growth and induces apoptosis and potentiated by bortezomib
- VernacularTitle:Bortezomib增强三氧化二砷抑制KM3细胞生长和促凋亡机制的研究
- Author:
Qing WANG
;
Juan LI
;
Jingli GU
;
Junru LIU
;
Lijin ZENG
- Publication Type:Journal Article
- Keywords:
As_2O_3;
Bortezomib;
Apoptosis;
Multiple myeloma;
KM3 cells
- From:
Chinese Journal of Pathophysiology
2010;26(2):297-301
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe if there is a synergistical effect on induction of apoptosis when arsenic trioxide alone or combination with bortezomib in KM3 cells. METHODS: KM3 cells were treated with arsenic trioxide alone or combined with bortezomib, the numbers of viable cells were determined by trypan blue exclusion. Cell growth inhibition was examined by MTT method. The cells were simultaneously stained with annexin V-FITC and PI and apoptosis was determined by bivariate flow cytometry using a FACScan. Reverse trascriptional-PCR (RT-PCR) method was used to examine the change of p65 mRNA and Western blotting to measure the expression of protein p65, p-p65, caspase-3, -8, -9, and poly ADP-ribose polymerase (PARP). RESULTS: Arsenic trioxide inhibited the cell growth and induced apoptosis. The mechanism was responsible for the activation of caspase-mediated induction of apoptosis. A synergistic effect of combination with bortezomib on apoptosis was observed. CONCLUSION: Arsenic trioxide inhibits KM3 cell growth and induces apoptosis with a synergistical effect when cotreated with bortezomib.
