High glucose induces release of endothelial microparticles in human umbilical vein endothelial cells and promotes cell apoptosis

Gang Tian; Yan Ren; Qun LU; Min LU

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High glucose induces release of endothelial microparticles in human umbilical vein endothelial cells and promotes cell apoptosis

VernacularTitle:高糖诱导人脐静脉内皮细胞内皮微粒释放并促进细胞凋亡
Author: Gang TIAN ; Yan REN ; Qun LU ; Min LU
Publication Type:Journal Article
Keywords: glucose; diabetes; atherosclerosis; endothelial microparticle; endothelial function
From:Journal of Xi'an Jiaotong University(Medical Sciences) 2010;31(2):181-185
CountryChina
Language:Chinese
Abstract: Objective To explore the effects of high glucose on release of endothelial microparticles (EMPs) and cell apoptosis in human umbilical vein endothelial cells (HUVECs). Methods HUVECs were incubated for 0h, 12h, 24h, 48h and 72h, respectively, with different concentrations of glucose (5.5mmol/L, 16.7mmol/L and 33.3mmol/L, respectively). The cells cultured in the media containing glucose 5.5mmol/L and mannitol 27.8mmol/L served as control groups. The survival rate of cells was examined by MTT assay. The level of EMPs release and percentage of cell apoptosis were detected by flow cytometry. Results ① According to MTT detection, the survival rate of HUVECs was 93% when incubated for 12h with 33.3mmol/L, 78% for 24h, 51% for 48h, and 40% for 72h. Compared with that for 0h, the survival rate for 12h did not decline significantly (P>0.05). the survival rate reduced significantly for 24, 48 and 72h, respectively, in the time-dependent manner (P<0.05). ② If the survival rate was 100% when incubated in 5.5mmol/L glucose for 48h, then it was 98% in mannitol 27.8mmol/L with no significant difference (P>0.05). It was 79% in 16.7mmol/L glucose and 51% in 33.3mmol/L glucose, obviously lower than that in 5.5mmol/L glucose, and the two groups differed significantly (P<0.05). ③ the level of EMPs release increased significantly in 33.3mmol/L glucose for 12, 24, 48 and 72h respectively, in the time-dependent manner. Compared with the normal glucose group, the level of EMPs release did not significantly differ in mannitol 27.8mmol/L for 12, 24, 48 and 72h , respectively (P>0.05). ④ There was a significantly positive correlation between EMPs release and cell apoptosis (r=0.659, P<0.05). Conclusion High glucose induces release of HUVECs endothelial miccroparticles and promotes cell apoptosis.