Effects of H_2O_2 on apoptosis of skeletal muscle satellite cell and mitochondrial membrane potential
- VernacularTitle:H_2O_2对大鼠骨骼肌卫星细胞凋亡和线粒体膜电位的影响及EPO的保护作用
- Author:
Linjie LUO
;
Jianghua LI
;
Shaohong DONG
;
Keqi CHEN
- Publication Type:Journal Article
- Keywords:
Hydrogen peroxide;
Skeletal muscle satellite cells;
Apoptosis;
Mitochondrial membrane potential;
Erythropoietin
- From:
Chinese Journal of Pathophysiology
2010;26(2):357-361
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe the effects of H_2O_2 on apoptosis of skeletal muscle satellite cells (SMSC)and mitochondrial membrane potential (MMP), and protective effect of erythropoietin(EPO). METHODS: SMSC in vitro were divided into three groups: H_2O_2 group, H_2O_2+EPO group and control. Apoptosis rate and the means were obverted by monofluorescence flow cytometry. The morphological change of apoptosis cells were observed under fluorescence microscopy after Hoechst 33258 staining. RESULTS: The cells in H_2O_2 group show the highest apoptosis rate (22.13±1.79)%. In H_2O_2+EPO group, apoptosis rate were (16.47±2.53)%, (4.97±0.55)% and (2.93±0.47)% according to the EPO treated levels (10, 20 or 40 kU/L), respectively. MMP level in H_2O_2 group was the lowest 9.70±0.09. MMP levels in H_2O_2+EPO group were 12.67±0.32, 27.90±0.66, 44.53±0.93, respectively according to the EPO treated levels (10, 20 or 40 kU/L). In control group, apoptosis rate was 1.93±0.57 and MMP was 51.37±0.64. In H_2O_2 group and H_2O_2+ low dosage EPO group, Hoechst 33258 staining showed obvious apoptosis. CONCLUSION: EPO inhibits the apoptosis induced by H_2O_2 and stabilizes the MMP, which is related to the dosage of EPO.
