SIGIRR overexpression inhibits LPS-induced NF-κB activation in alveolar epithelial cells
- VernacularTitle:SIGIRR过表达抑制LPS诱导的肺泡上皮细胞NF-κB的活化
- Author:
Feng TIAN
;
Jinbo ZHAO
;
Xiaofei LI
;
Tao JIANG
;
Yunjie WANG
- Publication Type:Journal Article
- Keywords:
Acute lung injury;
Single immunoglobin IL-1 receptor related protein;
Lipopolysaccharide;
NF-kappa B)
- From:
Chinese Journal of Pathophysiology
2010;26(2):379-383
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of single immunoglobin IL-1 receptor related protein (SIGIRR) on damage of alveolar epithelial cells in acute lung injury induced by lipopolysaccharide. METHODS: The acute alveolar epithelial cell injury model was constructed by stimulation of A549 cells with LPS. In order to over-express SIGIRR, the A549 cells were transferred with eukaryotic expression vector containing full length SIGIRR cDNA. The transcriptional activity of NF-κB was measured by dual-luciferase reporter assay system. The concentrations of IL-1β, TNF- α and IL-6 were detected by ELISA. The levels of these inflammatory factors between the transfected cells and untransfected cells were compared. RESULTS: The over-expression of SIGIRR inhibited the transcriptional activity of NF-κB. The increases in IL-1β, TNF-α and IL-6 concentrations in alveolar epithelial cells induced by LPS were observed. CONCLUSION: SIGIRR in alveolar epithelial cells inhibits TLR4 signals triggered by LPS and attenuates the inflammatory reactions in alveolar epithelial cells, which plays a protective role against the acute damage of the alveolar epithelial cells.
