Interleukin-7 influences the development of thymic T lymphocytes and thymic dendritic cells
- VernacularTitle:IL-7对胸腺T细胞及树突状细胞体外分化发育的影响
- Author:
Yunyun XU
;
Yong WANG
;
Yiping LI
;
Fang LI
;
Xiaoxia XU
;
Shoubing ZHU
;
Xueguang ZHANG
- Publication Type:Journal Article
- Keywords:
Fetal thymus organ cultures;
Interleukin-7;
Thymic T lymphocytes;
Thymic dendritic cells
- From:
Chinese Journal of Immunology
2010;26(2):108-112
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of IL-7 on differentiation of thymic T lymphocytes and dendritic cells' differentiation in vitro.Methods:Thymic lobes were apspectically removed from 15-day-old or 16-day-old mouse fetueses and were cultured by the way of fetal thymus organ cultures (FTOC) in vitro.Lobes were cultured in the dishes with or without Interleukin-7(IL-7),then collected the cultured cells respectively.The expression of CD4,CD8,CD11c,B220,Ia,and CD11b was detected by flow cytometry,and the morphology was observed under the ligt microscope.The proliferation of these cells was determined by cell counting.The cultured cells were used as stimulators for allogeneic T cells,whose proliferation were detected later by MTT method.Results:The result of cell counting indicated that the total amount of the thymocytes was decresed obviously after being cultured with IL-7,while the proportion of CD4~+ CD8~+ thymocytes decreased,the proportion of CD4~- CD8~-,and CD4~- CD8~+ thymocytes increased,and no much difference in the amount of CD4~+ CD8~- thymocytes was found.In addition,the amount of B lymphocytes,natural killer cells,and dendritic cells increased in different degree.Conclusion:IL-7 participates in differentiation and development of thymic T lymphocytes and dendritic cells.
