The influence of B-lymphocyte chemoattractant on the immune response of CVB3 fusion gene vaccine pcDNA3/C3d3-sVP1
- VernacularTitle:B细胞趋化因子对CVB3融合基因疫苗pcDNA3/C3d3-sVP1免疫效果的影响
- Author:
Zhiyun GAO
;
Xia CHUAI
;
Jiaming LAN
;
Guixia LIU
;
Jian LI
;
Yonghong ZHANG
;
Yongxiang WANG
- Publication Type:Journal Article
- Keywords:
B-lymphocyte chemoattractant;
Complement 3d;
Coxsackieviruses B3;
Gene vaccine
- From:
Chinese Journal of Immunology
2010;26(2):117-119
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the influence of B-lymphocyte chemoattractant on the immune response of CVB3 fusion gene vaccine pcDNA3/C3d3-sVP1.Methods:BALB/c mice were divided into 4 groups randomly, and injected intramuscularly with pcDNA3,pcDNA3/BLC,pcDNA3/C3d3-sVP1 and the combination with the plasmid pcDNA3/BLC and pcDNA3/C3d3-sVP1.At a certain time,they were measured for the titers for neutralizing antibodies,specific CTL cytotoxic activity.The protective efficacy of DNA vaccinations was evaluated by titers of blood viruses and survival rate.Results:The titers for antibodies increased with the time of inoculation.More specifically,the antibody titers (42.17±1.43) and the specific CTL cytotoxic activity (41.3%±3.51%) of the mice in the combination group were remarkably stronger than in the mice with pcDNA3/C3d3-sVP1(P＜0.05),but the virus titers of blood was lower.After lethal CVB3 challenge,the protection of mice from death in the combination group with the plasmid pcDNA3/BLC and pcDNA3/C3d3-sVP1 was 44%.Survival curves indicated that the survive state of combination group was better than others.Conclusion:BLC can strongly enhance the specific immunity induced by C3d3-sVP1.
