Production of discrepant monoclonal antibody against HL60 and HL60/ADR by SI technique
- VernacularTitle:消减免疫法制备抗HL60、HL60/ADR表面抗原差异抗体
- Author:
Simei REN
;
Yun YU
;
Ming SHE
;
Xiaofeng SHAO
;
Ruizan SHI
;
Hongwei PENG
;
Yang LIN
;
Xiuli ZHANG
;
Yanjun ZHANG
;
Dongsheng XIONG
- Publication Type:Journal Article
- Keywords:
Subtractive immunization;
Tumor drug resistance;
McAb;
Discrepant protein
- From:
Chinese Journal of Immunology
2010;26(2):160-163
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To prepare and characterize specific and discrepant mouse hybridoma antibodies on membrane of HL60 and HL60/ADR cell lines.Methods:BALB/c mice were immunized by subtractive immunization induced Cp(Cyclophosphamide).McAbs were prepared by hybridoma technique,screened and detected by FACS and LSCM.Results:51 candidates and discrepant antibodies were found,and one of them (5F6) was purified and identified.Conclusion:Combination of SI with discrepant screening method should facilitate the preparing and identifying discrepant McAbs for identifying antibodies that can distinguish the differences in proteins expressed in HL60 and HL60/ADR,which is a significative and potential method in the research and target therapy associated drug-resistance.
