Metformin down-regulates the expression of regulators of G protein signaling in OLETF rats

VernacularTitle:二甲双胍对OLETF自发性糖尿病大鼠G蛋白信号调节因子表达的影响
Author: Zongdong YU ; Jialin SU ; Kang LI ; Xujie ZHOU ; Guanping HAN ; Nana SONG ; Cheng CHEN ; Yumin DUAN ; Xiaohui GUO ; Yong HUO
Publication Type:Journal Article
Keywords: Regulators of G protein signaling; OLETF rat; Metformin; Diabetes mellitus,type 2
From: Chinese Journal of Diabetes 2010;18(1):54-56
CountryChina
Language:Chinese
Abstract: Objective To investigate the expression of regulators of G protein signaling(RGS), including RGS2, RGS3 and RGS4 in OLETF rats, as well as the effects of metformin on these expressions. Methods LETO rats were used as control group. Eight-week-old male OLETF rats were assigned to two guoups randomly:model and trial(metfomin dose during 8~(th) to 22~(nd) weeks:300mg kg~(-1)·d~(-1);during 23rd to 28th weeks:400 mg·kg~(-1) ·d~(-1))groups. Expressions of RGS mRNA in aorta and heart werequantified by real-time PCR. Results RGS2, RGS3 and RGS4 mRNA of the thoracic aorta and left ventricle were significantly higher in model group than in control group (P<0.01). Compared with model group, metformin significantly reduced their mRNA in trial group (P<0.01). Conclusions Upregulation of RGS2, RGS3 and RGS4 mRNA expression in the thoracic aorta and left ventricle of OLETF rats is in correlation with cardiovascular lesions; while downregulation of their expression is in correlation with the action of metformin.