Enhancive Effect of TNF-a on Transfection Efficiency of Ad-mediated Multidrug Resistance Gene1 (MDR1) in Mononuclear Cells of Mouse

VernacularTitle:TNF-α增强多药耐药基因1对骨髓保护的研究
Author: Wei LIU ; Qing LUO ; Xianqing JIN
Publication Type:Journal Article
Keywords: TNF-α; Multidrug resistance gene 1; Adenoviride
From: Chinese Journal of Clinical Oncology 2010;37(3):138-141
CountryChina
Language:Chinese
Abstract: Objective: To investigate the enhancive effect of TNF-α on transfection efficiency of adenovi-rus in mononuclear cells of mouse and the enhancive capability of protection of bone marrow by MDR1. Meth-ods: Before the mononuclear cells of mouse were infected by recombinant adenovirus encoding human MDR1 gene, they had been pretreated by TNF-α. Transfection efficiency of adenovirus was monitored by fluo-rescence microscopy, immunohistochemistry and flow cytometry (FCM). mRNA and protein levels of MDR1 in the mononuclear cells of mouse were detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot before and after treatment of TNF-α. Results: After treatment of TNF-α, tansfection rates of adenovirus were obviously increased in the treated group (26.26%) compared with the untreated group (11.96%). mRNA levels and protein levels of MDR1 were obviously increased in the treated group compared with the untreated group. Conclusion: TNF-α could enhance transfection efficiency of adenovirus in mononu-clear cells of mouse and enhance capability of protection of bone marrow by MDR1.