A Novel Electrochemical Enzyme-linked Immunosensor Based on Tyramine Signal Amplification
10.3724/SP.J.1096.2010.00258
- VernacularTitle:基于酪胺信号放大的新型免疫传感器
- Author:
Mengqin LIU
;
Jianhui JIANG
;
Yunlan FENG
;
Yong HUANG
;
Guoli SHEN
;
Ruqin YU
- Publication Type:Journal Article
- Keywords:
Electrochemical enzyme-linked immunosensor;
Signal amplification;
Tyramine oxidation deposition;
Biotin;
Gold nanoparticles
- From:
Chinese Journal of Analytical Chemistry
2010;38(2):258-262
- CountryChina
- Language:Chinese
-
Abstract:
A novel and highly sensitive voltammetric enzyme-linked immunosensor was developed based on tyramine) oxidation deposition. It was shown that gold nano-particles(colloid Au) could be used as a platform to immobilize antibodies by adsorption. By a sandwich immunossary format with goat-anti-human IgG labled Horseradish peroxidase(HRP) as the second antibody and catalytic amplification by biotin-tyramine, the immunosensor′s) catalytic ability to hydrogen peroxide increased nearly 20 times), the sensor exhibitd a linear response to human IgG in the concentration range from 1.5 μg/L-22 mg/L, and the detection limit was 0.1 μg/L), the regression equation could be expressed as Δi_p(μA) =2.8859c(mg/L)+17.152 with a correlation) coefficient of 0.9872. The immunosensor can be used to quantitatively determine hIgG in the sample) of human serum).
