Amperometric Immunosensor Based on Layer-by-layer Assembly of Thiourea and Nano-gold Particles on Gold Electrode for Determination of Naphthalene
10.3724/SP.J.1096.2010.00153
- VernacularTitle:多层自组装硫脲和纳米金电流型萘免疫传感器的研究
- Author:
Yan ZHANG
;
Huisheng ZHUANG
- Publication Type:Journal Article
- Keywords:
Label-free;
Amperometric immunosensor;
Thiourea;
Nano-gold particles;
Naphthalene
- From:
Chinese Journal of Analytical Chemistry
2010;38(2):153-157
- CountryChina
- Language:Chinese
-
Abstract:
A new type of amperometric immunosensor for the determination of naphthalene based on self-assemble) layer-by-layer technique was reported. The preparation procedure of such a sensor was characterized and the operation conditions, such as the pH value of supporting solution, incubated temperature and time were studied by cyclic voltammetry. Under the optimized working conditions, the current response of the immunosensor) was proportional to the concentration of naphthalene in the range of 0.5-100 μg/L with a detection) limit of 0.08 μg/L(r=0.9986). This method was applied to the naphthalene detection in river) water) samples and the recovery was ranging in 94.3%-107.0%. The reported immunosensor exhibited high sensitivity and long-term stability for naphthalene detection, and was easy to be prepared and regenerated.