Packed a Short Perphenylcarbamoylated β-Cyclodextrin Column for Rapid Enantioseparation of Five β-Blocker Drugs by High Performance Liquid Chromatography
10.3724/SP.J.1096.2010.00158
- VernacularTitle:高效液相色谱-全苯基衍生化β-环糊精短柱分离5种手性β-受体阻滞剂
- Author:
Haiping MA
;
Laisheng LI
;
Huiming CHEN
;
Yanhong JIN
- Publication Type:Journal Article
- Keywords:
High performance liquid chromatography;
Cycloderxtrin-bonded chiral stationary phase;
Channel-like SBA-15;
β-Blocker drugs;
Enantioseparation
- From:
Chinese Journal of Analytical Chemistry
2010;38(2):158-162
- CountryChina
- Language:Chinese
-
Abstract:
A method for the rapid enantioseparation of five β-blocker drugs, including alprenolol, propranolol), mexiletine, metoprolol and pindolol was developed by a 5-cm short column packed with perphenylcarbamoylated β-cyclodextrin(CD) chiral stationary phase by HPLC. The results showed that except for alprenolol), the other 4 β-blocker drugs were completely separated using ACN-0.1% thriethylammonium acetate(TEAA), 40∶ 60, V/V, pH=4.0) as mobile phase. The 5-cm short CD-based column exhibited rapid separation ability to the above β-blocker drugs within 5 min, which indicated that the separation has high efficiency. According to the chemical structures of the β-blockers and their chromatographic behavior, related separation mechanisms were also discussed. The proposed method was rapid, effective and repeated.
