Preparation and Characterization of Butylaminopropyl Silica Monolithic Column for Capillary Electrochromatography
10.3724/SP.J.1096.2010.00192
- VernacularTitle:丁基胺丙基硅胶毛细管整体柱的制备及其电色谱性能研究
- Author:
Fanggui YE
;
Yanyan HAN
;
Shun WANG
;
Baojun HUANG
;
Shulin ZHAO
- Publication Type:Journal Article
- Keywords:
Capillary electrochromatography;
Silica monolithic column;
Sol-gel;
Mixed mode
- From:
Chinese Journal of Analytical Chemistry
2010;38(2):192-196
- CountryChina
- Language:Chinese
-
Abstract:
A novel silica monolithic stationary phase functionalized with butylaminopropyl ligands for capillary electrochromatography(CEC) has been presented. The monolithic capillary columns were prepared by a sol-gel) process and subsequent a chemical modification. The amino groups on the surface of the stationary phase are meant to generate a substantial anodic electroosmotic flow (EOF). The butyl and propyl groups provide) hydrophobic properties. To evaluate the column performance, effects of buffer pH and organic modifier content on the EOF and electrochromatographic retention behavior of alkylbenzenes, organic acids and anilines were investigated. The monolithic stationary phase exhibited reversed phase (RP) chromatographic behavior toward neutral solutes. The model organic acid anion solutes were separated by the mixed mode mechanism, which comprised RP interaction, weak anion-exchange, and electrophoresis. Basic compounds such as anilines) were well separated on the butylaminopropyl silica monolithic column without peak tailing.
