Preparation and Application of Fluorescein Isothiocynate Fluorophore Nano-composites
10.3724/SP.J.1096.2010.00202
- VernacularTitle:异硫氰酸荧光纳米复合粒子的研制与应用
- Author:
Nianchun ZHANG
;
Yanhong GAO
;
Chuiming WAN
;
Yingliang LIU
- Publication Type:Journal Article
- Keywords:
Fluorescein isothiocyanate;
Fluorescein isothiocyanate and 3-aminopropyltriethoxysilane;
Nano-composites;
Bovine serum albumin
- From:
Chinese Journal of Analytical Chemistry
2010;38(2):202-206
- CountryChina
- Language:Chinese
-
Abstract:
Nano-composite fluorescence with silica shell had been prepared by improving St(o)ber route. The precursor was obtained via chemical reaction of fluorescein isothiocyanate(FITC) and 3-aminopropyltrietho-)xysilane(APTEOS)). Then, the controlled hydrolysis and condensation of tetraethylorthosilicate(TEOS) leaded) to the formation of organic-inorganic nano-composites. The transmission electron microscope(TEM) showed that the nano-composites with a diameter of about 70 nm were uniform, spherical and mono-dipersed. The nano-composites fluorescence significantly reduced dye leaching and had strong fluorescence when they were by water for several times. Labeled bovine scrum albumin(BSA) with the nano-composites fluorescence showed green fluorescence measured by laser scanning confocal microscope.
