Tandem Mass Spectrometry Acquisition Strategy Based on Exclusion of Precursor Ions
10.3724/SP.J.1096.2010.00241
- VernacularTitle:基于母离子排除的串联质谱数据采集方法的研究
- Author:
Wei MI
;
Jing WANG
;
Wantao YING
;
Wei JIA
;
Yun CAI
;
Xiaohong QIAN
- Publication Type:Journal Article
- Keywords:
Liquid chromatography-electrospray ionization mass spectrometry/mass spectrometry;
Precursor ion exclusion list;
Tandem mass spectrometry acquisition
- From:
Chinese Journal of Analytical Chemistry
2010;38(2):241-244
- CountryChina
- Language:Chinese
-
Abstract:
Due to the complexity of proteome samples, comprehensive analysis to characterize all proteins was still not possible with present methodologies. It has been shown that replicate runs could increase the number of identified) proteins. However, the redundancy of protein identifications was high. High-abundant peptides tended to be analyzed repeatedly in different runs. To reduce the redundancy and improve the efficiency of identification), we studied the MS/MS acquisition method of linear ion trap Fourier transform ion cyclotron resonance)-mass spectrometry(LTQ-FT) and an acquisition strategy based on exclusion of precursor ions was developed). It proved that the strategy could extremely reduce the redundancy of MS/MS acquisition and improve) the efficiency of protein identifications.
