Immunosensor Based on Immobilizing Antibody of Aflatoxin B_1 Using Silica Sol-Gel Technology
10.3724/SP.J.1096.2010.00245
- VernacularTitle:溶胶凝胶法固定抗体制备黄曲霉毒素免疫传感器
- Author:
Xiulan SUN
;
Zhongyun WANG
;
Yinjun FANG
;
Peipei CHEN
;
Zaijun LI
- Publication Type:Journal Article
- Keywords:
Aflatoxin B_1;
Ιmmunosensor;
Εlectrochemical impedance spectroscopy;
Sol-gel
- From:
Chinese Journal of Analytical Chemistry
2010;38(2):245-248
- CountryChina
- Language:Chinese
-
Abstract:
In the presence of hydrochloric acid, tetraethoxysilicane was hydrolyzed and formed silica sol. Non-labeled immunosensor was fabricated by droping the mixture solution of the silica sol and antibody of aflatoxin B_1 on the surface of glassy carbon electrode. In this work, a Fe(CN)_6~(3-/4-) phosphate buffer solution) was employed as base solution for investigating cyclic voltammetry(CV) and electrochemical impedance spectroscopic(EIS) performances of the sensor, respectively. The experimental results t indicated that because of the complex formed by the immunoreaction hindered the diffusion of Fe(CN)_6~(3-/4-) on the electrode surface, the redox peak current of the immunosensor in CV obviously decreased, and its electron transfer impedance linearly) increased with increasing the concentration of aflantoxin B_1(AFB). When the medium acidit and incubation) time were pH 6.5 and 20 min, respectively, the biggest electron transfer impedance changed value before and after the immunoreaction was obtained. Under the optimal conditions, a linear range to concentration of aflatoxin B_1 was 1-10 μg/L with a detection limit of 0.1 μg/L(S/N=3). Proposed method is of high sensitivity and stability, it has been successfully applied to determine AFB_1 in maize, rice and peanut.
