Up-regulation of DNA-PKcs and Its Mechanism in Human Glioma

VernacularTitle:非同源末端连接修复通路DNA-PKcs基因在胶质瘤中表达及其机制研究
Author: Zhixiang ZHUANG ; Liqin SHEN ; Shuyu ZHANG ; Peng QIU
Publication Type:Journal Article
Keywords: Glioma; Non-homologous end joining; DNA-PKcs; Methylation; Real-time PCR
From: Chinese Journal of Clinical Oncology 2010;37(4):216-219
CountryChina
Language:Chinese
Abstract: Objective: To detect the gene expression of Ku70, Ku80, ERCC4, lig4 and DNA-PKcs in non-homologous end joining pathway in human pdmary glioma tissues and normal brain tissues and to explore the underlying mechanism. Methods: The expression levels of Ku70, Ku80, ERCC4, lig4 and DNA-PKcsin in 36 glioma samples and 12 normal brain tissue samples were measured by SYBR Green real-time quantitative PCR. Methylation of DNA-PKcs was detected by methylation-specific PCR (MSP). Results: There was no significant difference in Ku70, Ku80, ERCC4 and lig4 expression between human primary glioma and normal brain tissues (P<0.05), while DNA-PKcs was significantly up-regulated (P= 0.002). The expression of DNA-PKcs was significantly higher in grade Ⅲ and Ⅳ glioma than that in grade Ⅱ glioma and normal brain tissues (P<0.05). Moreover, glioma tissues showed weaker methylation than normal brain tissues. Conclusion: The up-regulation of DNA-PKcs may be associated with pathogenesis of glioma. Demethylation of DNA-PKcs promoter is an important reason for its up-regulated expression in glioma.