The Affection on Cochlea Function of Guinea-pig by Transplanting Controlled Release BMP-2 into the Middle Ear
- VernacularTitle:缓释骨形态发生蛋白-2听泡内植入对豚鼠内耳功能的影响
- Author:
Xuesheng LI
;
Jianjun SUN
;
Wei JIANG
;
Xiao LIU
- Publication Type:Journal Article
- Keywords:
Prothesis;
Bone morphogenetic protein;
Cochlea;
Hearing threthold
- From:
Journal of Audiology and Speech Pathology
2010;18(1):39-42
- CountryChina
- Language:Chinese
-
Abstract:
Objective The controlled release BMP-2 and implantation into the acoustic bulla of the guineapig and the effects on the cochlea were observed.Methods The acellular cancellous bone was prepared,accompanied with BMP-2.The material accompanied with BMP-2 was implanted into one acoustic bulla of the animal,and the opposite side of the acoustic bulla was implanted with acellular cancellous bone without BMP-2.A total of 20 guinea-pigs were undergone this procedure.The ABR was tested on the animals before and after the operation immediately and 3 month after operation,respectively.3 month after operation,the animals were sacrificed.The osteogenesis induced by BMP-2,the acoustic bulla and the cochlea affected by BMP-2 were observed.The structures of hair cells were observed after being treated with silver nitrate.Results The animals recovered soon after surgery.The hearing thresholds of the animals reduced slightly after the operation,and recovered completely 3 month after.The bulla and the cochlea were normal in shape.The osteogenesis occurred in the pore of the acellular cancellous bone with BMP-2.No abnormal hyperplasia of bone in the hulla and the cochlea was found.The articulation between the stapes and oval window was not merged.The shape of the hair cells was normal and no obvious deletion of the hair cells was noted when compared with control group.Conclusion The controlled release BMP-2 could induce osteogenesis in the bulla of the animals.This material did not affect the shape of the bulla and the hearing thresholds.They did not induce any abnormal hyperplasia of bone in the bulla and might be used to reconstruct the affected ossicles.
