The Expression of Growth Associated Protein-43 in Inferior Colliculus in Rats after Bilateral Cochlear Ablation
- VernacularTitle:大鼠双侧耳蜗毁损后下丘核生长相关蛋白表达研究
- Author:
Yuankun GAO
;
Qingquan HUA
;
Hua LIAO
;
Meng LI
- Publication Type:Journal Article
- Keywords:
Auditory plasticity;
Cochlear ablation;
GAP-43
- From:
Journal of Audiology and Speech Pathology
2010;18(1):48-51
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expression of growth associated protein-43(GAP-43)in the inferior colliculus in rats following bilateral cochlear ablation.Methods 35 healthy adult SD rats were randomly divided into 7 groups:3,7,14,21,28,90 days following bilateral cochlear ablation and the sham operation group as the control group.The immunohistochemistry was used to observe the expression of GAP-43 after bilateral cochlear ablation in each group.Results GAP-43 was expressed in each group.The GAP-43 expression in bilateral cochlear ablation group were higher than that in the control group.The expression increased at 3 days,followed by a constant increase at 7 to 14 days with the peak on day 14.On day 21 the GAP-43 expression began to decrease.The mean gray level of GAP-43 in all the staining of groups had statistic significance compared to the control group except the group of day 90.However,in all the 7 groups,the mean gray level between the left and right ears had no statistic significance.Conclusion The change of GAP-43 expression in the inferior colliculus following bilateral cochlear ablation reflects synaptic plasticity in inferior colliculus the neurons.
