Differentiation of bone marrow mesenchymal stem cells into chondrocytes induced by transforming growth factor beta 1
10.3969/j.issn.1673-8225.2010.01.009
- VernacularTitle:转化生长因子β1诱导骨髓间充质干细胞向软骨细胞分化
- Author:
Liyan LI
;
Jinzhong HUANG
;
Jiang DU
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2010;14(1):38-41
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: Transforming growth factor beta 1 (TGF-β1) is a first selected growth factor in study of articular cartilage tissue engineering. Suitable concentration can stimulate proliferation, division and differentiation of articular chondrocytes. OBJECTIVE: To establish an special inducing system for differentiation of bone marrow mesenchymal stem cells (BMSCs) into chondrocytes in contained TGF-β1 culture in vivo,and to observe the changes in cell form and phenotype.METHODS: BMSCs were separated and purified from rabbit tibial tubercle using attachment culture method. Surface antigen of the third-generated BMSCs was determined by using flow cytometry. Subsequently,the third-generated BMSCs were induced with TGF-β1-contained special inducing system in 21-day culture, which was compared with human septal cartilage cells of nose following induction. Collagen type Ⅱ was qualitatively determined using immunohistochemical method.RESULTS AND CONCLUSION: Rabbit BMSCs were separated and purified using attachment culture method. The third-generated BMSCs were positive for surface antigen CD44,but negative for surface antigen CD34 and CD45. Cell form was irregular 21 days after induction. Immunohistochemical staining for type II collagen demonstrated positive cells. Results suggested that under culture system containing TGF-β1 BMSCs may directionally differentiate into chondrocytes,and there were not significant differences with normal chondrocytes.
