Basic fibroblast growth factor-induced differences in gene expression of human umbilical cord blood CD34~+ and CD133~+ stem cells: Gene chip analysis
10.3969/j.issn.1673-8225.2010.01.016
- VernacularTitle:利用基因芯片分析碱性成纤维细胞生长因子诱导脐血干细胞CD34~+与CD133~+细胞基因表达的差异
- Author:
Huiping CHEN
;
Qianru LI
;
Jing ZHANG
;
Ying DU
;
Bo YANG
;
Guoxi LI
;
Xiang HU
;
Ziming DONG
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2010;14(1):75-81
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: Further studies are needed to understand the cytobiological character, functional regulation, gene changes and expression difference of CD34~+ and CD133~+ stem cells induced by basic fibroblast growth factor (bFGF) using gene chip. OBJECTIVE: To compare the differences of gene expression and the response to bFGF of human umbilical cord CD34~+ and CD133~+ cells, and to explore gene expression changes of bFGF-induced umbilical cord CD34~+ and CD133~+ hematopotic stem cells/hemapoietic progenitor cells in vitro. METHODS: Human umbilical cord blood CD34~+ and CD133~+ cells were isolated and purified by MiniMACS immunomagnetic beads selection. The CD34~+ and CD133~+cells were cultured for 10 to 15 days in DMEM/F12 medium, supplemented with bFGF and B27. Total RNA from these cells was extracted and the genetic level of these cells was performed using Oligo GEArray(r) chip and GEArray software. Selected rate of CD34~+ and CD133~+ hematopoietic stem cells was detected using flow cytometry. CD34~+ and CD133~+ cell morphological changes were measured before and after bFGF induction. The concentration and purity of RNA were determined by agarose gel electrophoresis degeneration. Gene-chip test results were analyzed.
