In vitro and in vivo models of osteosarcoma for research of enhanced green fluorescent protein expression
- VernacularTitle:表达增强型绿色荧光蛋白的小鼠骨肉瘤体外和体内荷瘤模型的建立
- Author:
Xiao YANG
;
Jiang HU
- Publication Type:Journal Article
- Keywords:
enhanced green fluorescent protein;
transfection;
S180
- From:
China Oncology
2010;20(1):27-30
- CountryChina
- Language:Chinese
-
Abstract:
Background and purpose: Mouse osteosarcoma model was widely used in osteogenic malignant tumor research, and it was helpful for studying the invasion and metastasis of the tumor cells when it was well marked in vivo. The purpose of this study was to establish mouse sarcoma cell lines (S180) that were infected with enhanced green fluorescent protein(EGFP). Methods: EGFP-S180 expressing strong EGFP fluorescence was acquired by electroblot, and supplemented with G418 (800 mg/mL), c-Myc was detected by laser scanning confocal microscopy. Meanwhile, the cancer-bearing model was established subcutaneously within the abdominal cavity. Results: EGFP-S 180 cells were cloned. There was no significantly difference between c-Myc expressions in S180 cells and those in EGFP-S180 cells (P>0.05), and between the cancer-bearing time subcutaneously and the time within abdominal cavity (P>0.05). Conclusion: According to in vitro and in vivo assay, it showed that EGFP-expressing S180 cells could be used for studying further the tumor biological behavior with fluorescence technology.
