Study on construction of citrostatin and its bioactivity
- VernacularTitle:靶向抗肿瘤肽的构建及其生物学活性研究
- Author:
Li MA
;
Zailong CAI
;
Qingrong WANG
;
Chengxiang LEI
- Publication Type:Journal Article
- Keywords:
citrostatin;
fused peptide;
antineoplastic drugs;
antiangiogenesis
- From:
Chinese Journal of Biochemical Pharmaceutics
2010;31(1):19-22
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To construct a recombined antitumor peptide and to analyze its bioactivity. Methods Constructing a recombined gene and inserting the pGEX-4T-3 vector. The recombined protein was expressed in E. coli BL21 and purified with Amylose Resin. Then, citrostatin was subjected to the following tests separately: inhibition of endothelial cell proliferation, MTT test of cytotoxicity and inhibition of endothelial cell tube formation on ECMatrix. Results Citrostatin significantly inhibited the proliferation of human endothelial cell ECV304(IC_(50) = 2.28 μmol/L) .It also significantly inhibited the proliferation of human tumor cell 1990 and NCI-H64O(IC_(50) = 9.24,2.74 μmol/L) ,and the inhibitory effect became more marked with the increase of citrostatin concentration. The inhibitory effects of citrostatin on endothelial cell tube formation was also confirmed . Conclusion An antitumor peptide, citrostatin, has been successfully constructed and purified, which showed anti-angiogenesis effect and direct cytotoxic effect on tumor cells.
