Preparation of bFGF-PLGA controlled-release microspheres and their sustained release performance in vitro
- VernacularTitle:bFGF-PLGA缓释微球及其体外释药性能研究
- Author:
Jimin WU
;
Pengfei WANG
;
Zhihong LI
;
Xiaoyan YUAN
- Publication Type:Journal Article
- Keywords:
bFGF;
PLGA microspheres;
sustained release
- From:
Chinese Journal of Biochemical Pharmaceutics
2010;31(1):32-34
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To prepare bFGF-PLGA microspheres and to investigate the characteristics. Methods The bFGF-PLGA microspheres were prepared by W_1/O/W_2 multiple emulsion volatilizing method, the morphology was investigated using scanning electron microscope (SEM), the ELISA method was used to establish the regression equation and to detect the drug loading amount and encapsulation efficiency, as well as sustained-release profile in vitro . Results The microspheres seemed to be smooth and uniform with mean particle size of (0.75 ±0.08) μm,the the drug loading amount and encapsulation efficiency were [(59.9± 1.9) × 10~(-3)] % and (79.9±2.8)%, respectively, the accumulative release ratio was up to 80 % in the continuous period of forty-five days. Conclusion The bFGF-PLGA microspheres have better pharmaceutical properties and long-time sustained release effect in vitro.
