Determination of the activity of anti-F Ⅹ a and anti-F Ⅱ a of heparin by micro-chromogenic substrate methods
- VernacularTitle:微量生色底物法测定肝素钠抗Ⅹa因子和抗Ⅱa因子活性
- Author:
Jing LI
;
Huihong FAN
- Publication Type:Journal Article
- Keywords:
heparin;
anti-FⅩ a activity;
anti-F Ⅱ a activity;
chromogenic substrate method
- From:
Chinese Journal of Biochemical Pharmaceutics
2010;31(1):45-47
- CountryChina
- Language:Chinese
-
Abstract:
Purpose Semi-macro-chromogenic substrate methods have been adopted by British Pharmacopoeia to determine the anti-F Ⅹ a activity and anti-F Ⅱ a activity of low molecular weight heparin. Based on the above methods, the micro-chromogenic substrate methods were established to determine the anti-F Ⅹ a and anti-F Ⅱ a activity of heparins. Methods After the adjustment of the concentration of reagents and the whole volume of the test, 96-well microplates and the microplate spectrophotometer have been used to determine the absorbance. Results Six samples of heparin's potencies of anti-F Ⅹ a and anti-F Ⅱ a have been determined. The test results show that the errors of the tests are small and the repeatability is good. Conclusion Micro-chromogenic method can be used to determine the activity of anti-F Ⅹ a and anti-F Ⅱ a of heparins. This method improved the efficiency of the test and decreased the cost of the test. Mow the chromogenic substrate methods are being considered as the pharmacopoeia methods to substitute the anticoagulant methods.