An improved method for isolation of mouse islets and islet transplantation
- VernacularTitle:小鼠胰岛的分离及胰岛移植
- Author:
Xiaoping QI
;
Baoli MA
- Publication Type:Journal Article
- From:
Chinese Journal of Organ Transplantation
2001;22(3):176-178
- CountryChina
- Language:Chinese
-
Abstract:
Objectives To study of the method for isolation and transplantation of mouse islets. Methods The method for isolation of mouse islets described by Gotoh G was modified. The solution of digestion was injected not through the common bile duct but through gallbladder. Soybean trypsin inhibitor and BSA were added into the digestive and Ficoll separation solutions. Results The yield of islets was increased from 41.7±13.2 to 266.5±32.1(P<0.01). Islet viability was more than 95!%. Among the purified islets, there was no exocrine tissue but few ductal fragments. Conclusions By the improved method, digestive solution could be injected into pancreas without inverted microscope, which made manipulation easier and more successful. Having avoided the digestive effect of trypsin on islets, the yield of islets was increased and good repetitiousness was obtained.
