Toxicity and biocompatibility of polyethylene glycol-polyethyleneimine/ferroso-ferric oxide nano-magnetic fluid
10.3969/j.issn.1673-8225.2010.03.016
- VernacularTitle:聚乙二醇-聚乙烯亚胺/四氧化三铁纳米磁流体的毒性及其生物相容性
- Author:
Fen ZOU
;
Yifeng PAN
;
Hong ZHANG
;
Guozheng LI
;
Jingbo TANG
;
Jian PENG
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2010;14(3):447-451
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: Polyethylene glycol-polyethyleneimine/ferroso-ferric oxide (PEG-PEI/Fe_3O_4) was selected as drug carders in tumor treatment, which can increase drug loading capacity and targeting capacity.OBJECTIVE: To test the toxicity of PEG-PEI/Fe_3O_4 nano-magnetic fluid in vitro and in vivo. METHODS: When the prepared PEG-PEI/Fe_3O_4 nano-magnetic fluid reached nano level, 7702 and HpG2 cell lines were filtrated and diluted in 5-20 multiple, and detected by in vitro MTT toxicity test assay; in vivo hemolysis test and micronucleus test was used to test the toxicity and biocompatibility.RESULTS AND CONCLUSION: MTT assay results indicated that the toxicity grade of PEG-PEI/Fe_3O_4 nano-magnetic fluid to 7702 cell line was 0-1, which was harmless to natural hepatic cells; however, PEG-PEI/Fe304 nano-magnetic fluid had slight bystander restraining effect to HpG_2 cell line. Maximum hemolysis rate of the matedel was 0.372%, which was far less than 5%. The micronucieus test result indicated that PEG-PEI/Fe_3O_4 nano-magnetic fluid had no teratogenicity or mutagenicity.
