Preparation of chitosan/polyvinyl alcohol composite scaffold at different mass ratios: Comparison of structure and characteristics
10.3969/j.issn.1673-8225.2010.03.023
- VernacularTitle:不同质量配比壳聚糖/聚乙烯醇复合材料组织工程支架的制备:结构及性能比较
- Author:
Qinhua LI
;
Lei HUANG
;
Hui LI
;
Sihan FANG
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2010;14(3):477-481
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: Polyvinyl alcohol (PVA) has similar water content as human tissues, good biocompatibility and high mechanical strength, thereby being utilized as tissue-engineered matrix material. However, the modification in cellular affinity benefits the function as tissue regeneration scaffold materials. OBJECTIVE: To prepare chitosan (CS) / PVA composite scaffold and explore the feasibility of serving as a scaffold for tissue engineering. METHODS: Scaffolds of CS/PVA was fabricated by blending PVA-124 with various amounts of CS using film forming, particle forming, and free-drying methods. Water content, light transmittance, and swelling ratio were tested. Scanning electron microscope was used to observe the configuration of the cress section. RESULTS AND CONCLUSION: A total of 7 different proportional scaffolds were obtained with different PVA and CS dosage. The composite film showed light transmittance between 70%-80%, high water content between 121.2%-162.5% and swelling ratio between 60.3%-133.7%.Composita particle and sponge obtained water content between 82.0%-461.2% and 280.8%-1 939.0%. Composite scaffold with the amount ratio of PVA 0.75 g/CS 0.15 g exhibited the best performance. Scanning electron microscope showed that at a mass ratio of PVA 0.75 g/CS 0.15 g, the scaffold prepared by freeze-drying method had regular, tluffy inner structure, with good mechanics and high water content.
