Cellular compatibility of modified poly-lactic glycolic acid/type-Ⅰ collagen compound scaffold and rabbit ear chondrocytes
10.3969/j.issn.1673-8225.2010.03.001
- VernacularTitle:改性聚乳酸-羟基乙酸/Ⅰ型胶原复合支架与免耳软骨细胞的细胞相容性
- Author:
Binghua ZHOU
;
Wenbo LIAO
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2010;14(3):381-384
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: Poly-lactic glycolic acid (PLGA) is a promising cell scaffold material. However, its hydrophilicity and cellular affinity is poor, so it is necessary to modify its performance.OBJECTIVE: To explore the hydrophilic ability of the modified PLGA scaffold compounded with type Ⅰ collagen, and the cellular biocompatibility with chondrocyte of rabbit ear.METHODS: PLGA scaffold was modified with Poly-L-Lysine and compounded with type I collagen. The gross structure of scaffold was observed by inverted microscopy. The modified PLGNtype-Ⅰ collagen scaffold (experimental) and PLGA scaffold (control) were immerged in distilled water for 0.5, 1, 2, 4, 8, 12 and 24 hours. Chondrocytes were cultured by enzyme digestion method, and the second passage cells were seeded on surfaces of two scaffolds. Cell morphology was observed by phase contrast microscopy; cell attachment rate 24 hours after seeding was calculated, and the cell proliferation was determined by MTT assay at 1, 2, 4 and 6 days.RESULTS AND CONCLUSION: Modified composite scaffold exhibited high porosity and increased surface roughness compared with control group. Water uptake of two scaffolds displayed statistically significance at the same time point (P < 0.01), indicating the modification improved the hydrophilicity. The attachment rate of chondrocytes was 0.908 0+0.019 2 in modified compound scaffolds and 0.733 2±0.047 5 in control scaffold after 24 hours (P < 0.05), indicating the improved cellular affinity following modification. After 1, 2, 4 and 6 days, the absorbance between two groups was significantly different (P < 0.05), indicating the modified scaffold improved cell proliferation.
