CCR7 implications of spleen dendritic cells in multiple organ dysfunction syndrome in mice
- VernacularTitle:CCR7在多器官功能障碍综合征小鼠脾脏树突状细胞迁移变化中的作用
- Author:
Guocun HOU
;
Jiangyang LU
;
Hongwei WANG
;
Qian LIU
;
Guang TIAN
;
Yi YANG
;
Shaoran LI
- Publication Type:Journal Article
- Keywords:
Multiple organ dysfunction syndrome(MODS);
CCR7;
Spleen;
Dendritic cell
- From:
Chinese Journal of Immunology
2009;25(12):1063-1066
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore CCR7 expression in splenic dendritic cells and its role in migration and activity of splenic dendritic cells in multiple organ dysfunction syndrome (MODS) in mice.Methods:The MODS model of mice was reproduced by Zymosan injection into peritoneal cavity.The mice were randomly divided into groups of normal,3-6 hours,24-48 hours and 10-12 days post zymosan injection.CD11c and CD205 were analysed by immunohistochemistry;The expression of CD86 and CCR7 of DCs were studied by the flow cytometry analysis.Results:In normal mice,many DC were found at the margin between the red and white pulp.In the 3-6 h and 24-48 h groups,CD86 and CCR7 were strongly up-regulated in the DC,and they distributed in T cells areas.In the 10-12 d group,DC distributed at the margin by the immature form.Conclusion:The CCR7 expression level of DC has close correlations with the migration of DC,CCR7 can be used to evaluate the migration and functional activity of DC in MODS.
