Establishment of an immunoassay method for Osteogenic Growth Peptide
- VernacularTitle:成骨生长肽免疫检测方法的研究
- Author:
Yi XIAO
;
Jianguo WANG
;
Lanlan LIU
;
Baojun WU
;
Zengliang BAI
- Publication Type:Journal Article
- Keywords:
Osteogenic Growth Peptide;
Enzyme linked immununosorbent assay;
Osteoporosis;
Fracture healing
- From:
Chinese Journal of Immunology
2009;25(12):1116-1118
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To develop an enzyme immunoassay for the detection of Osteogenic Growth Peptide in serum for clinical investigation.Methods:Two kinds of competitive Enzyme linked immununosorbent assay were employed,based on sensitivity and shape of dose-reaction curve,the most suitable testing pattern was selected for further application.Results:Purified antibody was better than salting-out antibody in competitive Enzyme linked immununosorbent assay in terms of standard curve.Modified competitive Enzyme linked immununosorbent assay was better than competitive Enzyme linked immununosorbent assay in sensitivity.Conclusion:Modified competitive Enzyme linked immununosorbent assay for Osteogenic Growth Peptide is sensitive enough for clinical investigation.
