Biocompatibility of silk scaffold/3T3-L1 preadipocytes compounds:An in vitro experiment
10.3969/j.issn.1673-8225.2010.03.045
- VernacularTitle:蚕丝支架与3T3-L1前脂肪细胞生物相容性的体外实验
- Author:
Xiuhua LIU
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2010;14(3):563-566
- CountryChina
- Language:Chinese
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Abstract:
BACKGROUND: Silk, a natural product, has been paid wide attention in medical field owing to a fact that silk's mechanical property and biocompatibility are superior to traditional artificially synthesized degradable polymers. OBJECTIVE: To observe the effects of silk on the adsorption as well as cell morphology and function of 3T3-L1 preadipocytes. METHODS: Raw silk and trypsin-digested silk were ad Ubitum twisted into reticular fibrous cord with three-dimensional structure: mesh size 70-200 μm, thickness 200-300 μm, and porosity 20%. The three-dimensional scaffolds made of digested silk were placed in the 24-well plate. 3T3-L1 preadipocytes suspension (6×10~(10)/L) was added to the culture plate, 3 drops comprising 1 × 10~7 cells per well. Following 4-hour incubation in the air, when cells fully attached to the scaffold, they were thoroughly soaked with medium, which was renewed every other 2-3 days for a total of 1-4 weeks.RESULTS AND CONCLUSION: Inverted microscope results showed that silk scaffold/3T3-L1 preadipocytes compounds presented with slender prominences that stretched out and migrated ahead to gradually connect together through a head-to-end fusion fashion and enter into meshes. Scanning electron microscope results demonstrated that in the silk scaffold/3T3-L1 preadipocytes compounds, ceils tightly attached to silk scaffold, appropriately spread out, and secreted matrix. Silk shows better absorption for 3T3-L1 preadipocytes and maintains the normal morphology and functions of 3T3-L1 preadipocytes.
