Expression of vascular cell adhesion molecule 1 and intercellular adhesion molecule 1 in rat bone marrow mesenchymal stem cells
10.3969/j.issn.1673-8225.2010.06.038
- VernacularTitle:大鼠骨髓间充质干细胞血管细胞黏附分子1及细胞间黏附分子1的表达
- Author:
Yun XU
;
Yanlai HU
;
Zhaolin ZHANG
;
Yanhong LIANG
;
Hua TIAN
;
Guangping TIAN
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2010;14(6):1130-1133
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: Following bone marrow mesenchymal stem cells (BMMSCs) infusion therapy, which factor promotes BMMSCs migrated to correct position is a key point, currently, adhesion molecule is thought to be playing an important role in mediating BMMSCs migration. OBJECTIVE: To investigate the expression of vascular cell adhesion molecule 1 (VCAM-1) and intercellular adhesion molecule 1 (ICAM-1) in rat BMMSCs. METHODS: BMMSCs were in vitro separated from rat bone marrow by directly adherence method. The expression of VCAM-1 and ICAM-1 were identified by using immunocytochemical staining, and the expression rates of antigen were tested by flow cytometry, in addition, their mRNA expressions were measured by RT-PCR. RESULTS AND CONCLUSION: Immunocytochemistry demonstrated that BMMSCs weakly expressed VCAM-1, but strong expressed ICAM-1. Flow cytometry showed that the expression rate of VCAM-1 was 6%, and the expression rate of ICAM-1 was 100%. RT-PCR showed that BMMSCs expressed a low level of VCAM-1 mRNA but a high level of ICAM-1 mRNA. It revealed under physiological condition, BMMSCs expressed a low level of VCAM-1, whereas they expressed a high level of ICAM-1.
