Changes of vascular cell adhesion molecule-1 in serum and marrow stroma cell supernatant of patients with relapsed acute leukemia
10.3969/j.issn.1673-8225.2010.06.032
- VernacularTitle:复发性急性白血病患者血清和骨髓基质细胞培养上清液中血管细胞黏附分子1的变化
- Author:
Xuegang LI
;
Lijun HOU
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2010;14(6):1101-1104
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: Relapse of acute leukemia is possibly correlated with abnormal expression of cellular adhesion molecule-1 (VCAM-1).OBJECTIVE: To observe the level of VCAM-1 in serum and bone marrow stromal cell (MSC) supernatant of patients with relapsed acute leukemia. METHODS: Samples of serum and MSC supernatant were collected from 17 patients with remission-phase and relapse-phase acute leukemia hospitalized in the Department of Hematology, the Fifth Affiliated Hospital of Sun Yat-sen University between June 2006 and March 2008. The levels of VCAM-1 were measured with ELISA in remission and relapse phases.RESULTS AND CONCLUSION: Compared with the remission phase, the VCAM-1 level of serum was significantly increased in the relapse phase (P < 0.05). At 1 and 3 weeks after in vitro culture, the VCAM-1 level in the MSC supernatant was not changed (P > 0.05), but it was significantly increased on the fourth week (P < 0.05). The results demonstrated that VCAM-1 expression of serum and MSC supernatant in the relapse phase was greater than that in the remission phase, suggesting that abnormal expression of VCAM-1 was possibly correlated with relapse of acute leukemia.
