Construction of mouse interleukin-10 recombinant adenovirus vector and gene modification for dendritic cells
10.3969/j.issn.1673-8225.2010.05.021
- VernacularTitle:小鼠白细胞介素10重组腺病毒载体构建及对树突状细胞的基因修饰
- Author:
Fei XU
;
Chuanhui CHEN
;
Yaoguang LIN
;
Wei ZHANG
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2010;14(5):848-853
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: Few reports concern the effects of dendritic cells-a kind of antigen presenting cells, and interleukin-10 (IL-10) on airway hyperreactivity or inflammation. OBJECTIVE: To construct mice IL-10 recombinant adenovirus vector Ad-mIL-10 to acquire the dendritic cells modified by mIL-10, which can provide a foundation for the further study. METHODS: Mouse IL-10 (mIL-10) gene comprise of enzyme cutting spot was synthesized according to the mIL-10 gene sequence and multiclone spot of adenovirus vector, connected to pMD18-T vector and sequenced. MIL-10 was subcloned to BD Adeno-X~(TM) vector, packed and augmented in HEK 293 cells, following determine the protein expression, and the vector was transfected to mice bone marrow-derived dendritic cells. RESULTS AND CONCLUSION: Recombinant adenovirus vector Ad-mIL-10 was successfully synthesized, packed and augmented, which could highly express protein IL-10. Bone marrow-derived dendritic cells were successfully cultured and transduced in vitro. It suggested that it is feasible to transfect mice dendritic cells by Ad-mIL-10 adenovirus vector. The study can provide more sufficient theoretic evidence for the possibility of correlative gene therapy.
