In vivo Inhibition of NAS Preparation on H9N2 Subtype AIV

Author: Ruofeng SHANG ; Jianping LIANG ; Zhongyuan NA ; Hongjun YANG ; Yu LU ; Lanying HUA ; Wenzhu GUO ; Ying CUI ; Ling WANG
Publication Type:Journal Article
Keywords: NAS preparation; H9N2 subtype AIV; HA; RT-PCR
From: Virologica Sinica 2010;25(2):145-150
CountryChina
Language:Chinese
Abstract: NAS preparation, a kind of Chinese herbal medicine found by the Yunnan Eco-agricultural Research Institute, has potential antiviral activity. In this paper, the inhibiting effect of NAS preparation on H9N2 subtype Avian influenza virus (AIV) was investigated in vivo. Chickens infected with H9N2 virus were treated with NAS preparation for 4 days. The virus was then detected by hemoagglutination (HA) test and reverse transcription polymerase chain reaction (RT-PCR). The results showed that no H9N2 virus could be detected at the 7th day when the chickens were treated with 0.2g/kg/d or 0.1g/kg/d of NAS preparation. However the virus could be detected in other chickens without NAS preparation treatment. This result suggested that NAS preparation may be a potential drug candidate to control infection of H9N2 subtype AIV in chickens.