Effect of platelet-rich plasma on human periodontal ligament fibroblasts' proliferation, migration and differentiation
10.3969/j.issn.1001-3733.2010.02.013
- VernacularTitle:富血小板血浆对牙周膜成纤维细胞的增殖、迁移和分化的影响
- Author:
Liuxia SHI
;
Changping DI
;
Yan XU
;
Lu LI
;
Xiaoqian WANG
- Publication Type:Journal Article
- Keywords:
Periodontal ligament fibroblasts;
Platelet-rich plasma;
Transwell
- From:
Journal of Practical Stomatology
2010;26(2):194-197
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the in vitro effects of platelet-rich plasma(PRP) on human periodontal ligament fibroblasts(PDLFs). Methods: Various concentrations of PRP (10, 50, 100, 200, 300, 500 ml/L) were applied to primary cultures of human PDLFs. MTT assays were utilized to assess cell proliferation ability. Migration was determined by assessing the cell response to a concentration gradient with Transwell chamber. Differentiation was assessed using alkaline phosphatase (ALP) kit. Results: A beneficial effect on proliferation was observed, especially in response to 200 ml/L PRP.PRP had stimulatory effects on the migration of human PDLFs. PRP facilitated differentiation of PDLFs. Conclusion: PRP can exert a positive effect on human PDLFs,but this effect is concentration specific, while higher concentrations is not necessary to result in optimal outcomes.
