Establishment of rabbit femoral head necrosis models using liquid nitrogen, refrigeration method: Is it feasible, ideal and confident?
10.3969/j.issn.1673-8225.2010.02.020
- VernacularTitle:液氮冷冻法制作免股骨头坏死模型:可行,理想与可信
- Author:
Shuangwu DAI
;
Shuai DING
;
Zhanghua LI
;
Maokui WU
;
Jiangming XI
;
Fansheng MENG
;
Wenhua XIONG
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2010;14(2):276-279
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: The deficiency of perfect animal femoral head necrosis model limited its further investigation. OBJECTIVE: To verify the feasibility of establishing rabbit femoral head necrosis models using liquid nitrogen rsfdgeration method, and to provide a foundation for subsequent research. METHODS: A total of 20 adult, New Zealand, white rabbits were selected in the study. The round ligament of femur was not cut off and femoral head was not dislocated, and the exposed femoral head were quick frozen using cotton bud carrying liquid nitrogen for successive 25 times, with 10 s per time. The specimens were examined by gross anatomy, X-ray film, MRI and histological observation at day 3, 7 and weeks 2, 4, 6, and 8 after operation. RESULTS AND CONCLUSION: The histolOgical section showed that chondrocyte, osteccyts, and myelold tissues presented necrosis in freezing and periphery at 3days after model preparation, and the repair process appeared at 2weeks after operation. The articular surface of femoral heads appeared collapse at 4 weeks after operation, and these changes became obvious at 6 weeks. The femoral head presented ostecarthdtis-like disorder, with seriously collapsed articular surface at8 weeks, and the contour of femoral head changed in 2 animals. The results demonstrated that without hip dislocation, rabbit femoral head necrosis models can be established successfully using liquid nitrogen refrigeration method. This method is simple, feesible, with high succeed rate, which can be used in subsequent research.
