Isolation and cultivation of goat temporomandibular joint disc cells
10.3969/j.issn.1001-3733.2010.02.06
- VernacularTitle:山羊颞下颌关节盘细胞体外培养研究
- Author:
Weina SHU
;
Hong KANG
;
Weiping ZHANG
;
Xin LI
;
Guangjie BAO
- Publication Type:Journal Article
- Keywords:
One-month-old goat;
TMJ disc;
Cell culture;
Tissue engineering
- From:
Journal of Practical Stomatology
2010;26(2):165-168
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the isolation, culture and identification of the TMJ cells and to observe the biological characteristics of cultured fibrochondrocytes. Methods:The TMJ discs were dissected from two 1 month goats under sterile conditions and were digested with collagenase. The cells were collected. Morphological changes and attachment efficiency were constantly observed under phase-contrast microscope. Immunohistochemical staining for type I collagen as well as toluidine blue staining were performed. Ultrastructures of the TMJ cells were observed under transmission electron microscope. Results: Most of the primary fibrochondrocytes presented a short spindle-shape while the rest showed polygon-shape. On the 7th day, the perliferating fibrochondrocytes started to contact each other to form a monolayer covering the bottom of the incubation disc. Immunohistochemical staining of type I and toluidine blue staining exhibited positive results. The fibrochondrocytes cytoplasms were rich in mictochondria and endoplasm reticulum. Conclusion: The fibrochondrcytes isolated from one-month-old goat TMJ disc have good proliferation ability in vitro and cells from passage 1 to 3 might be used as seed cells for TMJ disc tissue engineering.
