An experimental study of BALB/c mice immunized by vaccine gene pcDNA3- PAc against dental caries through different routes
10.3969/j.issn.1001-3733.2010.02.01
- VernacularTitle:防龋基因疫苗pcDNA3- PAc不同途径免疫BALB/c小鼠的实验研究
- Author:
Zhaohui YUE
;
Jianguo LIU
;
Jing WANG
;
Jing ZHAO
;
Wei WANG
;
Jing JIANG
;
Xiaoyan GUAN
;
Jian ZHANG
- Publication Type:Journal Article
- Keywords:
Streptococcus mutans;
Surface protein antigen;
Gene vaccine;
Dental caries
- From:
Journal of Practical Stomatology
2010;26(2):145-148
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the anticaries effects of the vaccine gene pcDNA3- PAc against dental caries in BALB/c mice by different routes. Methods: BALB/c mice were immunized with the recombinant plasmid pcDNA3- PAc by the submandibular gland- target injection,the quadriceps femoris injection and the intranasal irrigation respectively. All the mice were immunized two times. The immune interval was two weeks. Saliva and serum samples were collected respectively at 0, 1, 2, 4 weeks after immunization. The specific antibodies were detected by ELISA. Results:The specific antibodies were up at one week after immunization in different routes. The peak time of the antibodies level appeared at 4 weeks.The level of salivary specific anti- PAc IgA induced by submandibular gland- target injection and that of serum IgG induced by thigh bone muscle injection was the highest, respectively. The differences of antibodies level between in experiment groups and negative control group or vacant comparison group were significant(P<0.01). Conclusion: The vaccine gene pcDNA3- PAc effectively induce local mucosal immune response and systemic immune response.
