Effect of extrinsic vascular endothelial growth factor (VEGF) on the viability of rat skin flap received radiotherapy

VernacularTitle:局部分次注射外源性VEGF影响大鼠放疗皮瓣成活性的实验研究
Author: Chunli LIU ; Yue WANG ; Zhihui LIU
Publication Type:Journal Article
Keywords: Vascular endothelial growth factor; Radiotherapy; Sink flap; Rat
From: Journal of Practical Stomatology 2010;26(2):169-172
CountryChina
Language:Chinese
Abstract: Objective:To observe the relationship between the vascular endothelial growth factor (VEGF) and the survival of flap in the rat back which received radiotherapy. Methods: Forty Wistar rats were divided into four groups. A 3×4 cm~2 full thickness dorsal flap with pedicle was prepared at the posterior end. The skin flaps were given~(60)Co radiotherapy in the 2, 4, 6 day postoperation. Group A was given of VEGF in the third, fifth, seventh day. Group B was given a high quantity of VEGF. Group C was the control of Group A,Group D was the control of Group B. Control groups received normal saline. Results: Pathological observation revealed that the survival of flap and blood vessel lumen diameter of group A were higher than that of group B(P<0.05). Group A showed remarkable difference compared with group C(P<0.01). Group B showed remarkable difference compared with group D(P<0.05). Results of fluorescence staining and SDH examination showed that three times injection had better results than one time injection. Conclusion: Three times injection of VEGF has better effect on the viability of rat skin flap than one time injection.