Establishment and characterization of a multidrug resistant human mucoepidermoid carcinoma xenograft model

VernacularTitle:人黏液表皮样癌裸鼠耐药移植瘤模型的建立及其生物学特性
Author: Xiaofang XU ; Bin LIU ; Junzheng WU ; Chunmei WANG ; Zhe WANG
Publication Type:Journal Article
Keywords: Mucoepidermoid carcinoma; Multidrug resistant; Nude mice
From: Journal of Practical Stomatology 2010;26(2):177-180
CountryChina
Language:Chinese
Abstract: Objective:To establish a multidrug resistant model of human mucoepidermoid carcinoma using nude mice. Methods:Multidrug resistant MEC/5-FU cells were inoculated intradermally into nude mice. Solid tumors were locally measurable after 10 days and 5-FU was repeated intraperitoneal injected into tumor-bearing mice. The tumor cells in nude mice (MEC/5-FU/NU) were isolated, cultured and examined. Results:The xenografts were similar to the original mucoepidermoid carcinoma from which the cell line was derived. The resistance index (RI) of the MEC/5-FU/NU cells to 5-FU was 27.82. Compared to the MEC, the expressions of ABCB1, ABCB11 and GSTA1 genes and MDR-1 protein increased in the MEC/5-FU/NU cells(P<0.05). Conclusion:The xenograft model is a good model of human multidrug resistant mucoepidermoid carcinoma, and may be useful in studying drug resistance mechanism in vivo.