Effect of GBE50 on L-type calcium current and cytosolic[Ca~(2+)]_i in ischemic guinea pig ventricular myocytes
- VernacularTitle:银杏酮酯对缺血豚鼠心室肌细胞I_(Ca-L)和游离钙的影响
- Author:
Aihua LIU
;
Zhixiong ZHANG
- Publication Type:Journal Article
- Keywords:
Ginkgo biloba extract 50;
ischemia;
ventricular myocytes;
whole-cell patch clamp;
L-type calcium current;
cytosolic[Ca~(2+)]_i
- From:
Chinese Pharmacological Bulletin
2010;26(3):329-333
- CountryChina
- Language:Chinese
-
Abstract:
Aim To observe the effect of Ginkgo biloba extract 50(GBE50)on L-type calcium current and cytosolic[Ca~(2+)]_i in ischemic guinea pig ventricular myocytes.Methods Single ventricular myocytes were isolated by enzymatic dissociation. I Ca-L was recorded by whole-cell patch clamp technique in voltage clamp mode.[Ca~(2+)]_i was detected by laser confocal micros-copy and represented by relative fluorescent intensity (FI).Results During ischemia, the peak Ca~(2+) current was reduced, and the I-U curve of I Ca-L was shifted upward.50 mg·L~(-1) GBE50 reversed the change induced by ischemia(n =6, P >0.05).After perfusing ischemic solution for 12 min, intracellular calcium concentration was increased(n =10, P <0.01).After perfusion with ischemic solution containing 50 mg·L~(-1) GBE50, the increase of intracellular calcium concentration was markedly inhibited(n =10, P >0.05).Conclusion GBE50 can reverse the decrease of I Ca-L and partially inhibit calcium overloading during ischemia.
