Effects of lipopolysaccharide on mice iron metabolism related genes mRNA expression
10.3969/j.issn.0529-1356.2010.02.011
- VernacularTitle:脂多糖对小鼠铁代谢相关基因mRNA水平表达的影响
- Author:
Xiaoqiang GUO
;
Yajuan MA
;
Sha TIAN
;
Hui DING
;
Guoqing REN
;
Yanzhong CHANG
;
Xianglin DUAN
- Publication Type:Journal Article
- Keywords:
Lipopolysaccharide;
Interleukin-6;
Hepcidin;
Ferroportin 1;
Transferrin receptor 1;
RT-PCR;
Rat
- From:
Acta Anatomica Sinica
2010;41(2):224-227
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effects of lipopolysaccharide (LPS) on mRNA expression of iron metabolism related genes. Methods Ten male mice (2 months) were injected intraperitoneally with lipopolysaccharide(0.5 μg/g). After 6 hours, mice were sacrificed and then sera, liver and spleen were collected. The mice blood routine was measured. The serum iron and total iron binding capacity (TIBC) were determined with reagent kit. The quasi-quantitative reverse transcription polymerase chain reaction (RT-PCR) was performed for mRNA of hepatic hepcidin(HP), ferroportin1(Fpn1), transferrin receptor 1(TfR1) and spleenic HP, Fpn1 and interleukin-6(IL-6). Results The serum iron and TIBC were reduced in mice injected LPS, which exhibited mild anemia(P＜0.05) . LPS can increase the expression of hepatic hepcidin and decrease Fpn1 and TfR1 in liver after LPS administration 6 hours(P＜0.05). In spleen, IL-6 was upregulated and Fpn1 downregulated(P＜0.05). Conclusion LPS can influence serum iron through regulating the mRNA expression of hepatic and spleenic iron metabolism related genes, such as HP, Fpn1 and TfR1.
