Triazole Schiff base derivative induces cannibalism of SMMC-7721 cells in vitro
10.3969/j.issn.0529-1356.2010.02.012
- VernacularTitle:三氮唑席夫碱衍生物诱导SMMC-7721细胞自相残
- Author:
Yusheng SUN
;
Chaoshen HUANGFU
;
Bin LIU
;
Yongchao MA
;
Guoqiang HU
- Publication Type:Journal Article
- Keywords:
Triazole Schiff base derivative;
Hepatocarcinoma cell;
Cannibalism;
Immunohistochemistry;
Transmission electronic microscopy;
Human
- From:
Acta Anatomica Sinica
2010;41(2):228-231
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo a nalyze the morphologic features of SMMC-7721 cannibalistic cells that induced by triazole Schiff base derivative(LH-37) in vitro. Methods The SMMC-7721 cells (1×10~4/ml)were cultured in the medium containing of 1×10~(-5) mol/L LH-37 for 24h,48h.The character of cells was detected by Papanicolaou and Wright′s Staining. Immunohistochemical method was used to observe the cleaved Caspase-3 positive cells. The ultrastructure of cannibalism cells was observed by JEM 100CX-II transmission electronic microscope. Results Microscopic analysis demonstrated the complete internalization of one cell within another. We noted that some cannibalistic cells in small aggregates appeared to be inside of large vacuoles, suggesting that they were internalized within a neighboring cell. The proportion of cannibalistic cells were increased after SMMC-7721 cells were cultured in the presence of LH-37 for 48 hours. The proportion of the cannibalistic cells in control and LH-37 group was 0.47% and 5.23% respectively . Many internalized cells were positive for cleaved caspase-3 staining . Ultrastructural analysis of engulfed cells from 24 hours exhibited evidence of live-cell internalization consistent with cannibalism, The most common fate for internalized cells was death after treatment with LH-37 for 48 hours, as evidenced by nuclear degradation and the eventual disappearance of some cells within the enveloping cell . Conclusion The data presented indicate that LH-37 can lead to an increase of cannibalism in human hepatocarcinoma cell in vitro.
