Expression and significance of TRH-R1 and TRH-R2 in the testis of EDS-treated adult rats
10.3969/j.issn.0529-1356.2010.02.017
- VernacularTitle:促甲状腺激素释放激素受体1和2在二甲磺酸乙烷处理大鼠睾丸中的表达及意义
- Author:
Huaping ZHU
;
Yong ZHAO
;
Jie ZHAO
;
Yuanqiang ZHANG
- Publication Type:Journal Article
- Keywords:
Thyrotropin-releasing hormone receptor;
Ethane dimethanesulphonate;
Testis;
Leydig cell;
Western blotting;
Immunohistochemistry;
Rat
- From:
Acta Anatomica Sinica
2010;41(2):252-256
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the expression of thyrotropin-releasing hormone receptor (TRH-R) type-1and type-2 in ethane dimethanesulphonate (EDS)-treated rat testis, and to discuss the significance of its expression in Leydig cells.Methods To make the injured testis Leydig cells rat model with EDS treatment. Western blotting, immunohistochemical ABC and immunofluorescence double labeling methods were used to detect the expression and location of TRH-R1 and R2 in the testicular tissues of EDS-treated-day 2,day 7,day 14,day 21 and day 28 rat mode, respectively. Results Western blotting results showed that the positive immunochemical staining was not found in the testicular tissues of the EDS-treated day 2 to day 14, on the other hand,they were found in EDS-treated-21 day and EDS-treated-28 day. Immunohistochemistry demonstrated that TRH-R1 and R2 expressed in the spindle-shaped cells reappeared around seminiferous tubules of post-EDS 21 days and 28 days groups. Immunofluorescence double labeling confirmed that these TRH-R1 and R2 positively stained cells were newly regenerated progenitor Leydig cells.Conclusion TRH-R1 and R2 are involved in the regeneration of Leydig cells in EDS-treated rat testis, and they may exert functions in the proliferation and differentiation of adult type Leydig cells.
