Effect of Schistosoma japonicum ova on NOD2/CARD15 during TNBS-induced colitis
- VernacularTitle:日本血吸虫卵对TNBS诱导小鼠结肠炎肠黏膜表达NOD2/CARD15的影响
- Author:
Chenmei XIA
;
Shuncai ZHANG
- Publication Type:Journal Article
- Keywords:
2,4,6-trinitrobenzesulfonic acid;
Schistosoma japonicum ova;
NOD2/CARD15;
inflammatory bowel diseases;
mice
- From:
Fudan University Journal of Medical Sciences
2010;37(2):162-166
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Schistosoma japonicum ova on the expression of intestinal NOD2/CARD15 in the mice induced by 2,4,6-trinitrobenzesulfonic acid (TNBS). Methods Mice (n=50) in the experiment were randomly allocated into 3 groups: control group (n=10), TNBS+saline (n=20) and TNBS+Schistosoma japonicum ova (n=20). TNBS enema (100 mg/kg) was applied to the two TNBS groups in order to establish a colonitis model. Schistosoma japonicum ova was administered i.p. on the 14~(th) and 3~(rd) day before the instillation of haptenating agent. All mice were killed on the 7~(th) day after colitis induction. The transcription level of NOD2 in colon tissues was measured by Real time PCR, and the expression of NOD2 protein was measured by Western blot. Results The transcription and protein levels of NOD2 in TNBS-induced mice increased statistically compared with those of the normal group (P<0.05). Compared with the TNBS-induced mice, Schistosoma japonicum ova-treated ones exhibited a statistical reduction of gene and protein expression (P<0.05). Conclusions TNBS-treated mice exhibited a statistical increased expression of NOD2/CARD15, Schistosoma japonicum ova treatment reduced the severity of experimental colitis through down-regulating NOD2/CARD15.
