Effects of cripto siRNA on liver metastasis of colorectal cancer cell in nude mice
- VernacularTitle:靶向cripto siRNA转染对裸鼠实验性大肠癌细胞肝转移的影响
- Author:
Ximing ZHONG
;
Yu FAN
;
Yongjing ZHOU
;
Jian CHEN
;
Gengjin LIN
- Publication Type:Journal Article
- Keywords:
colorectal carcinoma;
liver metastasis;
teratocarcinoma-derived growth factor-1;
small interfering RNA
- From:
Fudan University Journal of Medical Sciences
2010;37(2):202-206
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of cripto on migration, invasion, and liver metastasis of colorectal cancer cell. Methods After human colorectal cancer cell line SW480 was transfected by cripto small interfering RNA (siRNA), the mRNA and protein level were determined by Real-time RT-PCR and Western blot, respectively. The migration and invasion ability were evaluated by wound-healing assay and boyden chamber model, respectively. Thirty nude mice model of liver metastasis from colorectal cancer was established by splenectomy. Results The siRNA could down-regulate the level of mRNA and protein of cripto in a dose- and time-dependent manner. Suppression of cripto expression could inhibit migration and invasion ability of human colorectal cancer cell in vitro. The metastastic rate and tumor nodules were lower in transfection with cripto siRNA than in two control groups in vivo. Conclusions Cripto gene might play an important role in regulation of liver metastasis from colorectal carcinoma cell, and suppression of cripto gene by siRNA can inhibit liver metastasis of colorectal cancer.
